Overview

A lumbar puncture (spinal tap) is a test used to diagnose certain health conditions. It’s performed in your lower back, in the lumbar region. During a lumbar puncture, a needle is inserted into the space between two lumbar bones (vertebrae) to remove a sample of cerebrospinal fluid. This is the fluid that surrounds your brain and spinal cord to protect them from injury.

A lumbar puncture can help diagnose serious infections, such as meningitis; other disorders of the central nervous system, such as Guillain-Barre syndrome and multiple sclerosis; bleeding; or cancers of the brain or spinal cord. Sometimes a lumbar puncture is used to inject anesthetic medications or chemotherapy drugs into the cerebrospinal fluid.

Why it's done

A lumbar puncture (spinal tap) may be done to:

  • Collect cerebrospinal fluid to check for infections, inflammation or other diseases
  • Measure the pressure of cerebrospinal fluid
  • Inject spinal anesthetics, chemotherapy drugs or other medications
  • Inject dye (myelography) or radioactive substances (cisternography) into cerebrospinal fluid to make diagnostic images of the fluid's flow

Information gathered from a lumbar puncture can help diagnose:

  • Serious bacterial, fungal and viral infections, including meningitis, encephalitis and syphilis
  • Bleeding around the brain (subarachnoid hemorrhage)
  • Certain cancers involving the brain or spinal cord
  • Certain inflammatory conditions of the nervous system, such as multiple sclerosis and Guillain-Barre syndrome
  • Autoimmune neurological conditions
  • Alzheimer’s disease and other forms of dementia

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Risks

Although lumbar puncture (spinal tap) is generally recognized as safe, it does carry some risks. These include:

  • Post-lumbar puncture headache. As many as 25% of people who undergo a lumbar puncture develop a headache afterward due to fluid leaking into nearby tissues.

    The headache typically starts several hours up to two days after the procedure and may be accompanied by nausea, vomiting and dizziness. The headaches are usually present when sitting or standing and resolve after lying down. Post-lumbar puncture headaches can last from a few hours to a week or more.

  • Back discomfort or pain. You may feel pain or tenderness in your lower back after the procedure. The pain might radiate down the back of your legs.
  • Bleeding. Bleeding may occur near the puncture site or, rarely, in the epidural space.
  • Brainstem herniation. Increased pressure within the skull due to a brain tumor or other space-occupying lesion can lead to compression of the brainstem after a sample of cerebrospinal fluid is removed. To prevent this rare complication, a computerized tomography (CT) scan or magnetic resonance imaging (MRI) scan is often performed before a lumbar puncture to look for any sign of a space-occupying lesion resulting in increased intracranial pressure. A detailed neurological examination can also help rule out a significant space-occupying lesion.

How you prepare

Before your lumbar puncture (spinal tap), your health care provider takes your medical history, does a physical exam, and orders blood tests to check for bleeding or clotting disorders. Your provider may also recommend a CT scan or MRI to determine if you have any abnormal swelling in or around your brain.

Food and medications

Your health care provider will give you specific instructions about food, drink and medications.

Tell your provider if you're taking blood-thinning or other anticoagulant medications. Examples include warfarin (Jantoven), clopidogrel (Plavix) and apixaban (Eliquis). Also, tell your provider if you're allergic to any medications, such as numbing medications (local anesthetics).

What you can expect

A lumbar puncture (spinal tap) is usually done in an outpatient facility or a hospital. Your health care provider will talk to you about the potential risks, and any discomfort you might feel during the procedure.

If a child is having a lumbar puncture, a parent may be allowed to stay in the room in some cases. Talk to your child's health care provider about whether this will be possible.

Before the procedure

You may be asked to change into a hospital gown, although in some cases you may have the procedure while wearing your own clothing. There are a few possible positions for a lumbar puncture (spinal tap). Usually, you lie on your side with your knees drawn up to your chest, or you sit and lean forward on a stable surface. These positions flex your back, widening the spaces between your vertebrae and making it easier for your health care provider to insert the needle. Your back is washed with antiseptic soap or iodine and covered with a sterile sheet.

For an infant or a young child, someone will hold the child in position during the procedure.

During the procedure

  • A local anesthetic is injected into your lower back to numb the puncture site before the needle is inserted. The local anesthetic will sting briefly as it's injected.
  • A thin, hollow needle is inserted between the two lower vertebrae (lumbar region), through the spinal membrane (dura) and into the spinal canal. You may feel pressure in your back during this part of the procedure.
  • Once the needle is in place, you may be asked to change your position slightly.
  • The cerebrospinal fluid pressure is measured, a small amount of fluid is withdrawn and the pressure is measured again.
  • The needle is removed, and the puncture site is covered with a bandage.

The procedure usually lasts about 45 minutes. Your health care provider may suggest lying down after the procedure.

Sometimes, an ultrasound may be used as a guide during a lumbar puncture on infants and young children. The ultrasound can help prevent inserting the needle too far.

After the procedure

  • Plan to rest. Don't participate in strenuous activities the day of your lumbar puncture (spinal tap). You may return to work if your job doesn't require you to be physically active. Discuss your activities with your health care provider if you have questions.
  • Take a pain medication. A nonprescription pain-relieving medication that contains acetaminophen (Tylenol, others) can help reduce a headache or back pain. If your headache becomes severe, call your health care provider.

Results

The spinal fluid samples from the lumbar puncture (spinal tap) are sent to a laboratory for analysis. Lab technicians check for a number of things when examining spinal fluid, including:

  • General appearance. Spinal fluid is normally clear and colorless. If the color is orange, yellow or pink, it might indicate abnormal bleeding. Spinal fluid that is green might indicate an infection or the presence of bilirubin.
  • Protein (total protein and the presence of certain proteins). Elevated levels of total protein — greater than 45 milligrams per deciliter (mg/dL) — may indicate an infection or another inflammatory condition. Specific lab values may vary from medical facility to medical facility.
  • White blood cells. Spinal fluid normally contains up to five white blood cells per microliter. Increased numbers may indicate an infection or other condition. Specific lab values may vary from medical facility to medical facility.
  • Sugar (glucose). A low glucose level in spinal fluid may indicate an infection, tumor or another condition.
  • Microorganisms. The presence of bacteria, viruses, fungi or other microorganisms can indicate an infection.
  • Cancer cells. The presence of abnormal cells in spinal fluid — such as tumor or immature blood cells — can indicate certain types of cancer.

Lab results are combined with information obtained during the test, such as spinal fluid pressure, to help make a possible diagnosis.

Your health care provider generally gives you the results within a few days, but it could take longer. Ask when you can expect to receive the results of your test.

Write down questions that you want to ask your health care provider. Don't hesitate to ask other questions that may come up during your visit. Questions you may want to ask include:

  • Based on the results, what are my next steps?
  • What kind of follow-up, if any, should I expect?
  • Are there any factors that might have affected the results of this test and, therefore, may have altered the results?
  • Will I need to repeat the test at some point?

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April 30, 2022
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  3. Johnson KS, et al. Lumbar puncture: Technique, indications, contraindications, and complications in adults. https://www.uptodate.com/contents/search. Accessed Jan. 9, 2022.
  4. Fowler GC, et al., eds. Lumbar puncture. In: Pfenninger and Fowler's Procedures for Primary Care. 4th ed. Elsevier; 2020. https://www.clinicalkey.com. Accessed Jan. 9, 2022.
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  6. Graff-Radford J (expert opinion). Mayo Clinic. Jan. 31, 2022.

Lumbar puncture (spinal tap)