Immunodermatology Laboratory

By Mayo Clinic Staff

Studies based on immunohistologic staining have become indispensible in the evaluation of patients suspected of having immunologically mediated diseases of the skin or mucous membranes. Drs. A.L. Schroeter and R.E. Jordan, while residents in Dermatology at Mayo, were among the pioneers who recognized the value of immunofluorescence in the diagnosis of blistering diseases and who helped establish the application of this methodology for clinical testing in the management of cutaneous diseases.

The Immunodermatology Laboratory at the Mayo Clinic is a full service laboratory offering direct immunofluorescence of tissue and indirect immunofluorescence and immunoassay testing of sera for the evaluation of immunologically-mediated diseases. Reports include descriptive information summarizing the immunohistologic patterns, along with a diagnostic impression and, when appropriate, a comment. In addition, digital images of positive direct immunofluorescence staining results are provided to professional clients. Furthermore, our staff is available for interpretation of results via telephone, fax, or email.

Immunodermatology Laboratory Tests

Tests are available through Mayo Medical Laboratories.

Cutaneous Immunofluorescence
Biopsy (Tissue) Direct Immunofluorescence
The DIF test is performed on skin or mucosal biopsy specimens and is useful in the diagnosis of many immunodermatologic diseases.

Cutaneous Immunofluorescence Antibodies
Serum, Indirect Immunofluorescence (IIF)
The IIF test is performed on serum to detect the presence of circulating antibodies present in immunobullous disease.

Endomysial Antibody
Serum (EMA)
Circulating IgA endomysial antibodies (EMA) are present in 70 to 80 percent of patients with dermatitis herpetiformis and in nearly all patients with celiac disease who are not adhering to a gluten-free diet.

Tissue Transglutaminase(tTG) IgA Antibodies
Serum, Enzyme-Linked Immunosorbent Assay (ELISA)
Detection of these antibodies is an aid in diagnosis of certain gluten sensitive enteropathies associated diseases such as celiac disease and dermatitis herpetiformis and are useful in monitoring patient's adherence to a gluten free diet.

DSG 1 & DSG 3 ( ELISA)
This ELISA method detects and measures serum levels of antibodies of certain pemphigus diseases.

BP180 & BP230 (ELISA)
These ELISA methods are sensitive, objective, and specific tests that should be considered as an initial screening test in the diagnosis of pemphigoid and its variants.

Nov. 08, 2017