Fluorescent micrographs showing differentiated glioma cells growing as a monolayer in culture.

Fluorescent micrograph showing differentiated glioma cells growing as a monolayer in culture. Nuclei are stained dark blue. Turquoise, green, and red stains represent markers for oligodendroglial (O4), neuronal (β-tubulin), and astrocytic (GFAP) differentiation. Glioma stem cells express minimal glioneuronal differentiation markers, but these markers are highly expressed by differentiated glioma cells, a finding that demonstrates the ability of differentiated glioma stem cells to have pluripotency.