Fluorescent micrographs showing glioma stem cells growing as spheres in culture.

Fluorescent micrograph showing glioma stem cells growing as spheres in culture. Nuclei are stained dark blue. Turquoise, green, and red stains represent markers for oligodendroglial (O4), neuronal (β-tubulin), and astrocytic (GFAP) differentiation. Glioma stem cells express minimal glioneuronal differentiation markers, but these markers are highly expressed by differentiated glioma cells, a finding that demonstrates the ability of differentiated glioma stem cells to have pluripotency.