Laboratory Genetics in Minnesota

2005 Publications from the Cytogenetics Laboratory

2005 Publications
Abstracts
Books and Book Chapters

The following 2005 peer reviewed publications were produced by the Cytogenetics Laboratory and illustrate most of the areas that we have been investigating. As a laboratory, we published 40 papers, 52 abstracts, and 4 book chapters during 2005. The total career bibliography of the six laboratory directors combined totals 723 peer reviewed publications, 841 abstracts, 60 book chapters and 3 books that involve a wide variety of cytogenetic investigations.

2005 Publications

Adeyinka A, Adams SA, Lorentz CP, Van Dyke DL, Jalal SM. Subtelomere deletions and translocations are frequently familial. Am J Med Genet 135A:28-35, 2005.

Beauchesne LM, Warnes CA, Connolly HM, Ammash NM, Grogan M, Jalal SM, Michels VM. Prevalence and clinical manifestations of 22q11.2 microdeletion in adults with selected conotruncal anomalies. J Am Coll Cardiol 45:595-598, 2005.

Cassileth P, Lee S, Litzow M, Miller K, Stadtmauer E, Tallman M, Lazarus H, Bennett J, Paietta E, Dewald G, Rowe J.: Intensified induction chemotherapy in adult acute myeloid leukemia followed by high-dose chemotherapy and autologous peripheral blood stem cell transplantation: an eastern cooperative oncology group trial (E4995). Leukemia and Lymphoma 46: 55-61, 2005.

Caudill SP, Van Dyke DL, Chen ATL, Reidy JA, Ing PS, Schwartz S, Vance GH. Evaluating current policy for detecting mosaicism in amniotic fluid cultures: Implications for current cell counting practices. Statis Med 24:615-622, 2005.

Dewald GW, Therneau T, Larson D, Lee YK, Fink S, Smoley S, Paternoster S, Adeyinka A, Ketterling R, Van Dyke DL, Fonseca R, Kyle R. Relationship of patient survival and chromosome anomalies detected in metaphase and/or interphase cells at diagnosis of myeloma. Blood 106:3553-3558, 2005.

Dingli D, Mahaffey V, Spurbeck J, Reilly JT, Dewald GW, Tefferi A: Der(6)t(1;6)(q21-23;p21.3): The First Specific Cytogenetic Abnormality in Myelofibrosis with Myeloid Metaplasia. British Journal of Haematology 130: 229-232, 2005.

Eklund EA, Sun L, Yang SP, Pasion RM, Thorland EC, Freeze HH. Congenital disorger of glycosylation Ic due to a de novo deletion and an hALG-6 mutation: Biochem Biophys Res Commun. Epub ahead of print. 2005.

Elliott ME, Hanson CA, Dewald GW, Smoley SA, Lasho TL, Tefferi A: WHO-defined Chronic Neutrophilic Leukemia: WHO-defined Chronic Neutrophilic Leukemia: A Long-term Analysis of 12 Cases and a Critical Review of the Literature. Leukemia 19: 313-317, 2005.

Ensenauer RE, Shaughnessy WJ, Jalal, SM, Dawson DB, Courteau LK, Ellison JW. Trisomy 20 mosaicism caused by a maternal meiosis II error is associated with normal intellect but multiple congenital anomalies. Am J Med Genet 134A:202-206, 2005.

Finch R, Moore DG, Lindor N, Jalal SM, Markowitz A, Jhanwar S, Offit K, Guillem JG. Familial adenomatous polyposis and mental retardation by a de novo chromosomal deletion at 5q15-q22. Report of a case. Disease of the Colon & Rectum 48:2148-52, 2005.

Fink SR, Paternoster SF, Smoley SA, Flynn HC, Geyer SM, Shanafelt TD, Lee YK, Jelinek DF, Kay NE, Dewald GW: Fluorescent-labeled DNA Probes Applied to Novel Biological Aspects of B-cell Chronic Lymphocytic Leukemia. Leukemia Research 29: 253-262, 2005.

Galanis E, Buckner JC, Maurer MJ, Kreisberg JI, Ballman K, Boni J, Erickson BJ, Scheithauer BW, Jaeckle K, Peralba J, Jenkins RB, Dancey J, Hidalgo M, Walsh DJ: (NCCTG) Phase II Trial of temsirolimus (CCI-779) in recurrent glioblastoma multiforme. J Clin Oncol 23:5294-5304, 2005.

Jenkins RB , Adem C, Couch F, Hartmann LC: Reply to Palacios et al., "ERBB2 and MYC alterations in BRCA1- and BRCA2-associated cancers." Genes Chromosomes Cancer 42:206, 2005.

Kitange G, Misra A, Law M, Passe S, Kollmeyer TM, Maurer M, Ballman, Feuerstein BG, Jenkins RB: Chromosomal imbalances detected by array comparative genomic hybridization in human oligodendrogliomas & mixed oliastrocytomas. Genes Chromosomes and Cancer 42:68-77, 2005.

Lam JS, Yamashiro J, Shintaku IP, Vessella RL, Jenkins RB, Horvath S, Said JW, Reiter RE: Prostate stem cell antigen is overexpressed in prostate cancer metastases. Clin Cancer Res 11:2591-6, 2005.

Law M, Templeton K, Misra A, Kitange G, Smith J, Feuerstein B, Jenkins RB: A Molecular cytogenetic analysis of the chromosomes 1 and 19 in glioma cell lines. Cancer Genet Cytogenet 160:1-14, 2005.

Lewis JT, Ketterling RP, Halling KC, Reynolds CA, Jenkins RB, Visscher DV: Analysis of intratumoral heterogeneity and amplification status in breast carcinomas with equivocal (2+) Her-2/neu immunostaining. Am J Clin Pathol 124:273-281, 2005.

McClure RF, Remstein ED, Macon WR, Dewald GW, Habermann TM, Hoering A, Kurtin PJ: Adult B-Cell Lymphomas With Burkitt-Like Morphology Are Phenotypically and Genotypically Heterogeneous With Aggressive Clinical Behavior. Am J Surg Pathol 29:1652-1660, 2005.

McDonald JM, See SJ, Tremont I, Colman H, Gilbert M, Groves M, Burger P, Louis DN, Giannini C, Fuller G, Passe S, Blair H, Jenkins RB, Yang H, Ledoux A, Tipnis U, Zhang W, Hess K, Aldape K: Prognostic impact of histology and 1p status in anaplastic oligodendroglioma. Cancer 104:1468-1477, 2005.

Mistry S, Taylor C, Randerson-moor J, Harland M, Turner F, Barrett J, Whitaker L, Jenkins RB, Knowles MA, Bishop DT, Newton Bishop JA: Prevalence of 9p21 deletions in UK melanoma families. Genes Chromosomes Cancer 44:292-300, 2005.

Mora GR, Olivier KR, Mitchell RF Jr, Jenkins RB, Tindall DJ: Regulation of expression of the early growth response gene-1 (EGR-1) in malignant and benign cells of the prostate. Prostate 63:198-207, 2005.

Nowakowski GS, Dewald GW, Hoyer JD Paternoster SF, Stockero KJ, Fink SR, Smoley SA, Remstein ED, Phyliky RL, Call TG, Shanafelt TD, Kay NE, Zent CS: Interphase fluorescence in situ hybridization (FISH) with an igh probe is important in the management of patients with a clinical diagnosis of chronic lymphocytic leukemia (CLL). Br J Haematol 130: 36-42, 2005.

Pelloski CE, Mahajan A, Maor M, Chang EL, Woo S, Gilbert M, Colman H, Yang H, Ledoux A, Blair H, Passe S, Jenkins RB, Aldape KD: YKL-40 expression is associated with poorer response to radiation and shorter overall survival in glioblastoma. Clin Cancer Res 11:3326-34, 2005.

Perez EA, Sunman VJ, Rowland KM, Ingle JN, Salim M, Loprinzi CL, Flynn PJ, Malliliard JA, Kardinal CG, Krook JE, Thrower AR, Visscher DW, Jenkins RB. Two concurrent Phase II trials of Paclitaxel/Carboplatin/Trastuzumab (weekly or every-3-week schedule) as first-line therapy in women with HER2-overexpressing metastatic breast cancer: NCCTG Study 983252. Clinical Breast Cancer 6:425-432, 2005.

Qian X, Jin L, Shearer BM, Ketterling RP, Jalal SM, Lloyd RV. Molecular diagnosis of Ewing sarcoma/primitive neuroectodermal tumor in formalin-fixed paraffin-embedded tissues by RT-PCR and fluorescence in situ hybridization. Diagn Mol Pathol, 2005, 14:23-28.

Romond EH, Perez EA, Bryant J, Suman VJ, Geyer Jr. CE, Davidson NE, Tan-Chiu E, Martino S, Paik S, Kaufman PA, Swain SM, Pisansky TM, Fehrenbacher L, Kutteh LA, Vogel VG, Visscher DW, Yothers G, Jenkins RB, Brown AM, Dakhil SR, Mamounas EP, Lingle WL, Klein PM, Ingle JN, Wolmark N: Trastuzumab plus adjuvant chemotherapy for operable HER2-positive breast cancer. New Engl J Med 353:1673-1684, 2005.

Shearer BM, Flynn HC, Knudson RA, Ketterling RP. Interphase FISH to detect PBX1/E2A fusion resulting from the der(19)t(1;19)(q23;p13.3) or t(1;19)(q23;p13.3) in paediatric patients with acute lymphoblastic leukaemia. British J of Haematology, 2005, 129:45-52.

Shearer BM, Knudson RA, Flynn HC, Ketterling RP. Development of a D-Fish Method to detect DEK/CAN fusion resulting from t(6;9)(p23;q34) in patients with acute myelogenous leukemia. Leukemia, 2005, 19:126-131.

Sloma C, Hansen MJ, MacDougall AA, Van Keulen VP, Jenkins RB, and Pease LR: A class I transgene reveals regulatory events on chromosome 1 marking peripheral T cell differentiation and memory. J Immunol 174:7564-7572, 2005.

Steensma DP, Dewald GW, Lasho TL, Powell HL, McClure RF, Levine RL, Gilliland DG, Tefferi A: Brief Report: The JAK2 V617F Activating Tyrosine Kinase Mutation is an Infrequent Event in both "Atypical" Myeloproliferative Disorders and the Myelodysplastic Syndrome. Blood 106:1207-1209, 2005.

Stupca PJ, Meyer RG, Dewald GW: Using Controls for Molecular Cytogenetic Testing in Clinical Practice. J Assoc Genet Technol 31: 4-8, 2005.

Sweetser DA, Peniket AJ, Haaland C, Blomberg AA, Zhang Y, Zaidi ST, Dayyani F, Zhao Z, Heerema NA, Boultwood J, Dewald GW, Paietta E, Slovak ML, Willman CL, Wainscoat JS, Bernstein ID, and Daly SB. Delineation of the minimal commonly deleted segment and identification of candidate tumor suppressor genes in del(9q) acute myeloid leukemia. Genes Chromosomes and Cancer 44: 279-91, 2005.

Tefferi A, Dewald GW, Litzow ML, Cortes J, Mauro MJ, Talpaz M, Kantarjian HM. Chronic myeloid leukemia: current application of cytogenetics and molecular testing for diagnosis and treatment. Mayo Clin Proc 80:390-402, 2005.

Tefferi A, Dingli D, Li CY, Dewald GW: Prognostic diversity among cytogenetic abnormalities in myelofibrosis with myeloid metaplasia. Cancer 104: 1656-1660, 2005.

Velagaleti GVN, Bien-Willner GA, Northup JK, Lockhart LH, Hawkins JC, Jalal SM, Withers M, Lupski JR, Stankiewicz P. Position effects due to chromosome breakpoints that map similar to 900 Kb upstream and similar to 1.3 Mb downstream of SOX9 in two patients with campomelic dysplasia. Am J Hum Genet 76:652-62, 2005.

Wiktor AE, Van Dyke DL. Detection of Low Level Sex Chromosome Mosaicism in Ullrich-Turner Syndrome Patients. Am J Med Genet 138A:259-261, 2005.

Yang P, Kollmeyer TM, Buckner K, Bamlet W, Ballman KV, Jenkins RB: Polymorphisms in GLTSCR1 and ERCC2 are associated with the development of oligodendrogliomas. Cancer 103:2363-2372, 2005.

Yoong Y, Vandewalker TJ, Carlson RO, Dewald GW, Tefferi A.: Clinical correlates of submicroscopic deletions involving the ABL-BCR translocation region in chronic myeloid leukemia. Eur J Haematol 74:124-7, 2005.

Zabawski J, Wiktor A, Sikora M, Van Dyke DL. Use reference bands to accurately estimate ISCN band levels 400, 550, and 850. J Assoc Genet Tech 31:9-13, 2005.

Abstracts

Appelbaum FR, Kopecky KJ, Slovak ML, Gundacker HM, Tallman M, Kim H, Dewald GW, Estey E, Kantarjian H, Pierce S. The clinical spectrum of adult acute myeloid leukemia (AML) associated with core binding factor (CBF) translocations. Southwest Oncology Group, Seattle, WA; Eastern Cooperative Group, Philadelphia, PA; MD Anderson Cancer Center, Houston, TX. American Society of Clinical Oncology, Orlando, FL, May 2005. Abstract No: 653.

Buckner JC, Ballman KV, Scheithauer BW, Arusell RM, Blair HE, Passe AM, Brown PD, Jaeckle K, Shaw EG, Jenkins RB: NCCTG 94-72-53: Diagnostic and prognostic significance of 1p and 19q deletions in patients (pts) with low-grade oligodendroglioma and astrocytoma. (ASCO, 2005 Meeting).

Chhabra H, Thorland E, Lega M, Lewanda AF, Jalal SM. Characterization of an unusually large deletion involving the Williams syndrome region at 7q11.23 by use of BACs. Association of Genetic Technologists Annual Meeting, 2005.

Chng WJ, Van Wier S, Ahman G, Santana-Davila R, Jalal S, Ketterling R, Bergsagel PL, Chesi M, Trendle M, Oken M, Blood E, Henderson K, Kyle R, Gertz M, Lacy M, Dispenzieri A, Greipp P, Fonseca R. Compendium of karyotypic abnormalities and their clinical implications in hyperdiploid multiple myeloma (H-MM). American Society of Hematology 47 th Annual Meeting, Atlanta, Georgia, December 10-13, [1539] Blood 106 (Pt 1):442a, 2005 2005.

Courteau L, Lundquist, Ensenauer R, Jalal S, Dawson B. Detection of chromosomal 22q11 duplications using FlexMap ™ microspheres and MLPA. Association for Molecular Pathology Annual Meeting, Scottsdale AZ, 2005.

Dewald G , Fink S, Smoley S, Stockero K, Paternoster S, Schwager S, Thorland E, Van Dyke D, Shanafelt T, Zent C, Call T, Neil Kay N: Loss of p53 is Due to Rearrangements in a ~6,400 kb Region of Low Copy Repeats Near the Centromere of Chromosome 17 in Chronic Lymphocytic Leukemia (B-CLL). 47th Annual Meeting of American Society of Hematology. Atlanta, GA. December 2005. Blood 106: 910aa Abstract 3255, 2005.

Dewald GW : Validation of new assays and use of controls for FISH testing in clinical practice. Proceedings of the Forty-Third Annual Great Lakes Chromosome Conference. Toronto, Canada, May 20, 2005. Key note Address. Du Z, Nakagawa T, Kollmeyer T, Jenkins RB: Functional studies of candidate genes within the minimally overrepresented 8q24 region in prostate cancer. (Prostate SPORE, 2005 Meeting).

Einerson RR, Law ME, Blair HE, Kurtin PJ, McClure RF, Ketterling RP, Flynn HC, Allen JE, Dogan A, Remstein ED. 8q24 breakpoints associated with the t(8;22)(224.1;q11.2)/MYC/Immunoglobulin Lambda Light Chain Gene rearrangement in B-cell lineage malignancy are more widely dispersed than previously reported. American Society of Hematology 47 th Annual Meeting, New Orleans, Louisiana, December 3-6, 2005.

Ensenauer RE, Michels VV, Adeyinka A, Lindor NM, Dawson DB, Jalal SM. Microduplication 22q11.2: A new syndrome. Invited 45-minute platform presentation. American College of Medical Genetics, 2005.

Fernandez HF, Kim HT, Bennett JM, Dewald G, Tallman MS, Lazarus HM. Gemtuzumab-Ozogamicin (GO;Mylotarg (R)) as part of consolidation therapy for AML before autograft: low incidence of hepatic veno-occlusive disease (Abstract I87). Biol Blood Marrow Transplant 2005 Feb; 11(2 Suppl 1):62-63.

Fink S, Smoley S, Stockero K, Paternoster S, Schwager S, Thorland E, Shanafelt T, Zent C, Call T, Kay N, Dewald G: Loss of p53 is due to rearrangements in a ~6400 kb region of low copy repeats near the centromere of chromosome 17 in B-cell chronic lymphocytic leukemia. Abstracts of the XI International Workshop on CLL, New York, September 2005. Leukemia and Lymphoma 46 (1) 2005: P44.

Fonseca R, Van Wier SA, Chng WJ, Ketterling R, Lacy MQ, Dispenzieri A, Bergsagel PL, Rajkumar SV, Greipp PR, Litzow MR, Henderson KJ, Ahmann GJ, Gertz MA. Low level amplification of 1q21 in myeloma and prognosis: the role of CKS1B. American Society of Hematology 47 th Annual Meeting, Atlanta, Georgia, December 10-13, 2005.

Galanis E, Buckner JC, Maurer M, Hidalgo M, Kreisberg JI, Peralba J, Jenkins RB, Jaeckle K, Dancey J, Walsh DJ: N997B: Phase II Trial of CCI-779 in recurrent glioblastoma multiforme (GBM): Updated results and correlative laboratory analysis. (ASCO, 2005 Meeting).

Greipp PT, Fonseca R, Dewald G, Rajkumar SV, Morice WG, Greipp PR: Myeloma with t(11;14) and CD20+ plasma cells: response to rituximab. 47th Annual Meeting of American Society of Hematology. Atlanta, GA. December 2005. Blood 106: 5177a Abstract 5178, 2005.

Grinnell , SD , Stupca, PJ, Dewald, GW: Comparing band resolution by manual and density profile analyses. Association of Genetic Technologists Annual Meeting, Kansas City, KA, June 16-19, 2005. The Journal of the Association of Genetic Technologists: 31: A62

Hecksel KA, Dewald GW, Steensma DP: Ferrochelatase mutation screening by denaturing high performance liquid chromatography in idiopathic acquired sideroblastic anemia (refractory anemia with ringed sideroblasts and erythrocytic protoporphyria. 47th Annual Meeting of American Society of Hematology. Atlanta, GA. December 2005. Blood 106: 11b Abstract 3735, 2005.

Jenkins R: Molecular cytogenetic testing in neurooncology. (EANO/WFNO, 2005 Meeting)

Jenkins RB, Kollmeyer T, Buckner K, bamlet W, Ballman KV, Yang P: A GLTSCR1 Haplotype is associated with oligodendroglioma development. (EANO/WFNO, 2005 Meeting).

Keefe JG, Flynn HC, Ketterling RP. Development of a novel FISH assay to detect MLL/AF9 fusion associated with t(9;11)(p22;q23) in patients with acute leukemia. Association of Genetic Technologists 30 th Annual Meeting, Kansas City, Missouri, June 16-19, 2005.

Kirchhof A, James C, Nass B, Van Dyke D. Lean thinking: The miracle of doing more with less. Association of Genetic Technologists Annual Meeting, Kansas City, June 16-19, 2005.

Kitange GJ, Kollmeyer TM, Law M, Soderberg C, Templetom KL, Morlan BE, Ballman KV, Jenkins RB: Gene expression profile in glioma cell line A172 after microcell-mediated transfer of chromosome 19. (AACR, 2005 Meeting).

Knudson RA, Shearer BM, Flynn-Gilmer HC, Ketterling RP. FISH for the t(5;12)(q33;p13) (PDGFRbeta/TEL) seen in Acute and Chronic Myeloid Leukemias. Association for Molecular Pathology 11 th Annual Meeting, Scottsdale , Arizona , November 10-13, 2005.

List AF, Dewald G, Bennett J, Giagounadis A, Raza A, Feldman E, Powell B, Greenberg P, Zeldis J, Knight R: Hematologic and cytogenetic response to lenalidomide (CC-5013; REVLIMID ä ) in patients with transfusion-dependent (TD) myelodysplastic syndrome (MDS) and chromosome 5q31.1 deletion: Results of the multicenter MDS-003 study. American Society of Clinical Oncology, Orlando, FL, May 2005. Platform presentation. Abstract No: 5.

Mahaffey V, James C, Van Dyke DL. Metaphase scanning systems save time and gain efficiency. Association of Genetic Technologists Annual Meeting, Kansas City, June 16-19, 2005.

Moorman AV, Harrison CJ, Richards SM, Fielding A, Tallman MS, Litzow MR, Rowe JM, Goldstone AH, Dewald GW: Karyotype is an Independent Prognostic Factor in Adult Acute Lymphoblastic Leukaemia (ALL): Analysis of Cytogenetic Data from 1,235 Patients on the Medical Research Council (MRC) UKALLXII / Eastern Cooperative Oncology Group (ECOG) 2993 Trial. 47th Annual Meeting of American Society of Hematology. Atlanta, GA, December 2005. Blood 106: 100a Abstract 331, 2005.

Mota RA, Thorland EC, Jalal SM. Use of BACs and subtelomere FISH probes to detect a cryptic deletion at the breakpoint of a satellited 14q case. Association of Genetic Technologists Annual Meeting, 2005.

Nelson BP, Gupta R, Dewald GW, Paternoster S, Peterson LC. Feinberg Medical School, Northwestern University, Chicago, IL, Mayo Clinic, Rochester, MN. Utility of a FISH Chronic Lymphocytic Leukemia (CLL) Panel with Emphasis on the Significance of 14q32 Translocations. United States Canadian Academy of Pathologists. San Antonio, TX, Platform presentation, March 2005. [1124]

Nelson BP, Gupta R, Dewald GW, Paternoster SS, Peterson LC. Utility of a FISH chronic lymphocytic leukemia (CLL) panel with emphasis on the significance of 14q32 translocations (Abstract 1124). Laboratory Investigationist 2005 Jan; 85(Suppl 1):242A-243A.

Pardanani A, Ketterling R, Li CY, Wolanskyj A, Elliott M, Camoriano J, Butterfield J, Dewald G, Tefferi A: Low incidence of FIP1L1-PDGFRA in eosinophilic patients and long-term experience with imatinib therapy. 47th Annual Meeting of American Society of Hematology. Atlanta, GA. December 2005. Blood 106: 593a Abstract 2098, 2005.

Pestova E, Lucas M, Piper J, Ketterling R, Willie S, Piorunski L, Marble T, Perez T, Stenzel T, King W, Wilber K. A novel automated hybridization and improved data analysis method for aCGH arrays. American College of Medical Genetics Annual Meeting, Dallas, Texas, March 17-20, 2005.

Pestova E, Lucas M, Piper J, Ketterling R, Willie S, Piorunski L, Marble T, Perez T, Stenzel T, King W, Wilber K. Preliminary validation of improved analysis software and an automated hybridization method for a 333-clone CGH microarray. Association for Molecular Pathology 11 th Annual Meeting, Scottsdale , Arizona , November 10-13, 2005.

Rolig KA, Wiktor A, Lima J, Bloxham M, Lutterman M, Kuffel D, Anderson M, Jalal SM. Specimen processing changes results in significant time saving for prenatal, miscarriage and postnatal interphase FISH tests. Association of Genetic Technologists Annual Meeting, 2005.

Rowe JM, Li X, Cassileth PA, Appelbaum FR, Schiffer CA, Litzow MR, Cripe LD, Lazarus HM, Paietta E, Dewald GW, Weinstein HJ, Ogden AK, Woods WG, Shepherd L, Feusner JH, CBloomfield CD, Tallman MS: Very Poor Survival of Patients with AML Who Relapse after Achieving a First Complete Remission: the Eastern Cooperative Oncology Group Experience. 47th Annual Meeting of American Society of Hematology. Atlanta, GA. December 2005. Blood 106: 162a Abstract 546, 2005.

Schooley A, Van Dyke DL, McGrann PS. FISH for subtelomeres, TUPLE1, BCR, and ARSA reveal a complex mosaic karyotype in an 8-year old with a previously diagnosed R(22). Association of Genetic Technologists Annual Meeting, Kansas City, June 16-19, 2005.

Schop RFJ, Ocio EM, Gonzalez B, Gutierrez NC, Jesus Moro M, Aguilera C, Hernández J, García-Sanz R, Xu R, Hernandez-Rivas JM, Greipp PR, Dispenzieri A, Jalal SM, Lacy MQ, Gonzalez-Paz N, Gertz MA, San Miguel JF, Fonseca R: 6q Deletion in Waldenström Macroglobulinemia Is the Most Common Cytogenetic Abnormality and Is Associated with Aggressive Disease with a Trend towards Worse Survival. [986] Blood 106 (Pt 1):290a, 2005.

Shanafelt TD, Fink S, Witzig TE, Paternoster SF, Smoley S, Stockero K, Flynn HC, Almer C, Jenkins R, Geyer S, Kay NE, Dewald G: Frequency of Clonal Evolution by FISH in Untreated, Early Stage Patients with CLL: A Prospective, Longitudinal Study with Long Clinical Follow-up. 47th Annual Meeting of American Society of Hematology. Atlanta, GA. December 2005. Blood 106: 728a Abstract 2590, 2005.

Shearer B, Ketterling R. Evaluation of Spectral Genomics' Constitutional Chip™ 1.0 using microarray-based CGH detection of congenital chromosome anomalies in 46 patients: Are we there yet? American College of Medical Genetics Annual Meeting, Dallas, Texas, March 17-20, 2005.

Shearer BM, Flynn HC, Ketterling RP. Observance of PBX1/E2A gene fusion caused by the der(19) or t(1;19) in adult hematologic malignancies. Association for Molecular Pathology 11 th Annual Meeting, Scottsdale , Arizona , November 10-13, 2005.

Stevens R, Almanaseer I, Gonzalez M, Caglar D, Knudson RA, Ketterling RP, Bridge JA. Analysis of HER-2 gene amplification using an automated FISH signal enumeration system. Abstract 450427. United States and Canadian Academy of Pathology Annual Meeting,San Antonio, Texas, February 26-March 4, 2005.

Stockero K, Fink S, Smoley S, Paternoster S, Schwager S, Shanafelt T, Call T, Kay N, Dewald G: Submicroscopic interstitial deletions in 13q14 are detectable in metaphase cells by fluorescence in situ hybridization with D13S319 in B-cell chronic lymphocytic leukemia. Abstracts of the XI International Workshop on CLL, New York, September 2005. Leukemia and Lymphoma 46 (1) 2005: p50.

Stockero KJ, Fink SR, Paternoster SF, Smoley SA, Wiktor AE, Stupca PJ, Van Dyke DL, Dewald GW. How to validate FISH probes. Association of Genetic Technologists Annual Meeting, Kansas City, KA, June 16-19, 2005. The Journal of the Association of Genetic Technologists: 31: A4 Platform presentation.

Teece LL, Shearer BM, Oliveira AM, Llyod RV, Ketterling RP. Validation of a commercial FISH probe to detect rearrangements of the SYT Gene at 18q11.2 in synovial sarcoma. Association of Genetic Technologists 30 th Annual Meeting, Kansas City, Missouri, June 16-19, 2005.

Thorland EC, Shearer BM, Flynn HC, Hodnefield JM, Hoyer JD, Ketterling RP. Devlopment of an Interphase FISH Assay to Detect ABL1/CAN Gene Co-Amplification in Patients with T-cell Acute Lymphoblastic Leukemia. Association for Molecular Pathology 11 th Annual Meeting, Scottsdale , Arizona , November 10-13, 2005.

Thorland, EC, Shearer, BM, Flynn, HC, Adams, SA, Lindor, NM, Ketterling, RP, Chromosomal deletion of 15q15-21.2 in a patient with Marfan syndrome: implications for the mechanism of disease. American College of Medical Genetics. Abstract #169. Dallas, TX, 2005.

Van Dyke DL, Stockero K, Fink S, Smoley S, Paternoster S, Schwager S, Shanafelt T, Zent C, Call T, Kay N, Dewald G : Submicroscopic Interstitial Deletions in 13q14 are Detectable in Metaphase Cells by Fluorescence in situ Hybridization (FISH) with D13S319 in Chronic Lymphocytic Leukemia (B-CLL). 47th Annual Meeting of American Society of Hematology. Atlanta, GA. December 2005. Blood 106: 916aa Abstract 3278, 2005.

Vance G, Kim H, Hicks G, Cherry, Higgins R, Tallman MS, Fernandez HF, Dewald G : Utility Of Interphase FISH To Stratify Patients Into Cytogenetic Risk Categories At Diagnosis Of AML In An ECOG Clinical Trial (E1900). 47th Annual Meeting of American Society of Hematology. Atlanta, GA. December 2005. Blood 106: 669aa Abstract 2377, 2005.

Wiktor A, Fink SR, Stupca P, Stockero K, Majorowicz JR, Van Dyke DL, Dewald GW. Validation of FISH probes for clinical practice. American College of Medical Genetics Annual Meeting, Dallas, Platform presentation, March 17-20, 2005.

Wiktor AE, Thorland EC, Flynn HC, Van Dyke DL. FISH analysis to differentiate Type I and Type II deletions in Prader-Willi and Angelman syndrome patients. The American Society of Human Genetics, Salt Lake City, October 25-29, 2005.

Dingli D, Mahaffey VJ, Spurbeck JL, Dewald GW, Tefferi A: Der(6)t(1;6)(q21-23;p21.3): the most specific chromosomal translocation in myelofibrosis with myeloid metaplasia. American Society of Clinical Oncology, Orlando, FL, May 2005. Platform presentation. Abstract No: 6548.

Gliem T, Lega M, Jalal SM. An unusual bisatellited marker in a 9-year-old with anomalies. Association of Genetic Technologists Annual Meeting, 2005.

Merritt JL, Jalal SM, Barbaresi WJ. Research letter: 14q32.3 deletion syndrome with autism. Am J Med Genet 133A:99-100, 2005.

Adeyinka A, Kramer D, Van Dyke D. Loss of 17p is a major consequence of whole-arm chromosome translocations in hematologic malignancies. The American Society of Human Genetics, Salt Lake City, October 25-29, 2005.

Books and Book Chapters

Adeyinka A, Jalal SM. Clinical Applications of 24-colour FISH. In: Encyclopedia of Medical Genomics and Proteomics. Fuchs J, Podda M, editors. Marcel Dekker, New York City, 471-473, 2005.

Dewald GW, Ketterling, RP: Conventional Cytogenetics and Molecular Cytogenetics in Hematological Malignancies. In: Hematology: Basic principles and practice. Hoffman R, Benz E, Shattil S, Furie B, Cohen H, Silberstein L, McGlave P, editors, Churchill Livingston. Philadelphia, 53: 928-939, 2005.

Michels VV, Thibodeau SN, Thorland EC. Mendelian and Mitochondrial Inheritance, Gene Identification, and Clinical Testing. In Peripheral Neuropathy, 4th Edition, Dyck PJ and Thomas PK, editors. Elsevier Saunders, Philadelphia, 2:1545-1560,2005.

Wiktor A, Van Dyke DL. Cytogenetic Nomenclature, In: The Cytogenetics Symposia, second edition. Dunn B, Mouchrani P, Keagle M, editors. AGT Publications, 11-1 – 11-10, 2005